Morphological aspects and germ cells of male reproductive tract of river stingray, Potamotrygon amandae

The family Potamotrygonidae are the only species of stingrays restricted to fresh water and located exclusively in South America. objective this research was analyze morphological aspects germ cells male reproductive tract Potamotrygon amandae. samples were fixed 10% formalin, then dehydrated an ascending ethanol series (70 100%). To carry out light microscopy analyses, they embedded paraffin, cut stained; as for scanning electron dried, glued metallic bases metalized. gross morphology consisted following paired organs: testis, epididymis, deferent duct, Leydig gland, seminal vesicle, clasper, clasper gland. Microscopically, several stages spermatogenesis observed occurring spherical follicles, similar other stingrays. epididymis formed by one duct subdivided various tubules. ducts continuous with lumen full spermatozoa. glands glandular units eosinophilic content some, ran parallel ventral portion. vesicles possessed numerous compartments store sperm, a wall hive, Alcian Blue (AB) Periodic Schiff-Acid (PAS) performed Gland, deferens vesicle positive connective tissue, cilia PAS+ nuclei stained weakly AB. gland composed unit covered striated muscle externally. It very well Schiff-Acid. amandae